Ngwa Plasmid EndoFree Midi

Maka nsacha ngwa ngwa nke ọkara mkpụrụ ndụ ihe nfefe na-enweghị ọgwụ endotoxin DNA plasmid.

EndoFree Midi Plasmid Kit na -anabata teknụzụ mgbasa ozi silica pụrụ iche iji jikọta DNA plasmid nke ọma. Buffer pụrụ iche EBT na Filtration CS1 nwere ike wepu adịghị ọcha dịka endotoxin na protein. Usoro mwepụ ahụ dum na -ewe naanị elekere 1, nke dabara adaba yana ngwa ngwa.

Nwamba. Mba Nkwakọ ngwaahịa
4992853 10 nkwadebe

Nkọwa ngwaahịa

Usoro ọrụ

Nlereanya Nnwale

Mkpado ngwaahịa

Atụmatụ

Kwesịrị ekwesị maka iwepụta plasmids site na usoro ọdịbendị nje nje 50-100 ml.
Plas Plasmid ahụ dị ọcha nwere endotoxin dị ala nke ukwuu ma nwee ike mezuo ihe achọrọ maka mmụba dị elu.
Ọ dị mfe iji usoro mwepụ endotoxin pụrụ iche na ngwa ngwa rụọ ọrụ.

Ngwa

Enwere ike itinye DNA plasmid ahụ dị ọcha na nnwale bayoloji molekul na nnwale ihe ọmụmụ mkpụrụ ndụ dịka mmachi endonuclease mgbaze, usoro, mgbanwe, ntụgharị, microinjection, wdg.

Mmụba Plasmid

Yield

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)


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    Workflow

    Workflow Plasmid pEGFP-C1 bụ nke EndoFree Midi Plasmid Kit na-eme ka ọ dị ọcha site na 50 ml nke na-enye nje bacteria n'abali. Nanodrop tụrụ njupụta plasmid na gbakọọ mkpụrụ ya. Nsonaazụ gosiri na mkpụrụ plasmid sitere na EndoFree Mini Plasmid Kit dị elu karịa nke ahụ site na ngwaahịa dị mkpa sitere na Onye na -eweta A.
    Workflow PEGFP-C1 plasmid nke EndoFree Midi Plasmid Kit na-ebunye n'ime 80% confluence 293T sel site na TIANGEN TIANfect. Achọpụtara nkọwapụta nke GFP n'ime awa 24-36 mgbe emesịrị ya. Nsonaazụ gosiri na arụmọrụ ntụgharị ahụ bụ 80%-90%.
    Dee ozi gị ebe a ma zitere anyị ya