FastKing Otu Nzọụkwụ RT-qPCR kit

Otu nzọụkwụ Real-Time RT-PCR nwere SYBR-Green.

FastKing Otu Nzọụkwụ RT-qPCR Kit bụ maka Real-Time One Step RT-qPCR site na iji SYBR Green I. Enwere ike iji ngwa ahụ n'otu oge RT-qPCR nke ebumnuche RNA, yana ntụgharị ntụgharị na PCR na-ewere ọnọdụ n'usoro. n'otu tube. Ya mere ọrụ a dị mfe, ọ na -ebelata ihe ize ndụ nke mmetọ.

Ngwakọta 25 × RT-PCR Enzyme Mix na ngwa a bụ isi mmalite nke TIANGEN akwụkwọ mpịakọta transcriptase (FastKing RTase), antibody gbanwere hotstart. Taq DNA polymerase na onye na -egbochi RNase. FastKing RTase bụ ụdị ọhụrụ transcriptase nke molekụla gbanwere. Site na ebumnuche hydrophobic agbakwunyere, nke nwere njikọ RNA siri ike yana nkwụsi ike ọkụ. FastKing RTase emeela ka arụmọrụ transcript ntụgharị dị mma yana ikike ịgbatị ndebiri RNA nwere usoro nke abụọ dị mgbagwoju anya. Ụdị ọkụ ọkụ ọhụrụTaq A na -eji DNA polymerase nwere arụmọrụ dị mma na usoro PCR, na -eme mmeghachi omume PCR mgbe ntụgharị ntụgharị nwere arụmọrụ njupụta dị elu yana nkọwapụta ya. Na mgbakwunye, 2 × FastKing RT-qPCR Buffer (SYBR Green) na ngwaahịa a bụ sistemụ mmeghachi omume ọhụrụ kachasị maka enzymes abụọ dị n'elu, gụnyere ihe ndị dị mkpa ionic, dNTPs, SYBR Green dye, otu nkwụsi ike na nkwalite, nke nwere ike hụ na FastKing RTase na mmalite ọkụ ọhụrụTaq DNA polymerase nwere ike rụọ ọrụ kachasị dị irè na usoro mmeghachi omume otu nzọụkwụ niile.

Ngwa a na -enye akụkụ dị mma nke ọma n'ọtụtụ mpaghara ọnụọgụ. Ọ nwere ike chọpụta n'ụzọ ziri ezi na ọnụ ọgụgụ dị iche iche nke mkpụrụ ndụ ihe nketa lekwasịrị anya dị elu na nke dị ala nke nwere mmegharị dị mma yana ntụkwasị obi dị elu.