Ngwa nyocha HRM (EvaGreen)

Atụmatụ

Resolution Mkpebi dị elu: Nabata agba agba agba EvaGreen, nke nwere oke agbaze agbaze na steeti juru eju ma nwee ike ịmata ọdịiche nke otu ntọala.
Nkọwapụta dị elu: Jiri antibody gbanwetụrụ hotstart DNA polymerase iji belata nkwalite na-abụghị nke akọwapụtara ma melite nkọwapụta.
Stability Nkwụsi ike dị elu: Usoro akpachapụ anya na -ebuli usoro nchekwa ihe na -abawanye nkwụsi ike nke usoro agbaze ma melite ntụkwasị obi nke nsonaazụ.
Ction Ndozi ROX: A na -ekekọta ihe mkpuchi ROX iche iche, nke na -adị mfe iji ma nwee nsonaazụ ziri ezi karị.

Nkọwapụta

Ụdị: Antibody gbanwetụrụ Taq DNA polymerase, EvaGreen.
Ngwa: Amaara ederede SNP; Nyocha SNP amabeghị; Nnyocha mkpụrụ ndụ ihe nketa amaghi ama; Nyocha PCR Methylation.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

×

Enwetara DNA mkpụrụ ndụ ihe nketa site na 100 μl nke nlele ọbara mmadụ site na iji Kit DNA DNA. Ejiri 50 ng DNA maka nchọpụta PCR ozugbo. Dabere na ọba akwụkwọ NCBI SNP, ahọpụtara ma chọpụta SNP nke mkpụrụ ndụ FEN1 (RS 174538) site na iji Roche LightCycle480. Nsonaazụ ya bụ nke a:

Nsonaazụ gosiri na ngwa nyocha HRM bụ ezigbo ụzọ nyocha SNP mpaghara nwere nnukwu mmegharị nke agbaze agba nke otu genotype, mkpebi doro anya nke ụdị mkpụrụ ndụ ihe nketa dị iche iche na enweghị echiche ọ bụla.

Ozi SNP: …… CGGAAGAACACGTCG [A/G] CAGGAGCAGGCGCCT …… (Allele Frequency: A = 0.314, G = 0.686)

Primer: Maka iberibe bp 108 (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTTGGGTTGTG)