N’ọnwa Disemba afọ 2019, usoro ọrịa oyi baa n’amaghị ama kpatara bidoro na Wuhan, Hubei Province, na n'oge na -adịghị anya gbasaa n'ọtụtụ ógbè na obodo China, na ọtụtụ mba ndị ọzọ na Jenụwarị 2020. Ka ọ na -erule elekere 22:00 nke abalị na Jenụwarị 27, ikpe 28 enwetara na ikpe 5794 ndị a na-enyo enyo na 2019-nCov ekwupụtala na China. NdịEnwetara isi iyi nke nje dị ka Rhinolophus, ọnụ ọgụgụ ndị na -anwụ ugbu a bụ 2.9%.
Na Jenụwarị 12, 2020, Healthtù Ahụ Ike (wa (WHO) kwupụtara na ụdị ọrịa coronavirus ọhụrụ (2019-nCov) kpatara ọrịa oyi baa. Coronavirus bụ ụdị nje na -agbasa n'etiti anụmanụ. Ụdị nje nucleic acid bụ RNA nwere otu ebe. N'otu oge ahụ, WHO wepụtakwara usoro usoro nucleic acid nke nCov nke ndị ọkà mmụta China kesara, nke nyela ntọala nchọpụta mkpụrụ ndụ maka nchọpụta nje ma mee ka nchọpụta ngwa ngwa na ịchọpụta nje na -ekwe omume.
WHO na -atụ aro na a ga -enyocha ndị mmadụ nwere nnukwu ọrịa iku ume dịka ụkwara, ahụ ọkụ, ọrịa akụkụ okuku ume na ndị gara Wuhan n'ime ụbọchị iri na anọ ma ọ bụ bụrụ ndị ekpughere na kọntaktị ndị ọrịa ndị ọzọ. Na Jenụwarị 17, 2020, WHO wepụtara “nnwale ụlọ nyocha maka akwụkwọ akụkọ coronavirus 2019 (2019-nCoV) na ikpe mmadụ na-enyo enyo, ntuziaka nwa oge, 17 Jenụwarị 2020”. Ntụziaka ahụ rụtụrụ aka na ihe nlele a ga -anakọta n'aka ndị ọrịa nwere akara ngosi gụnyere ụdị iku ume (nasopharynx na swabs oropharyngeal, sputum, lavage bronchoalveolar, wdg) na ụdị ọbara, dị ka ndị a:
*Maka ibupu nlele maka nchọpụta nje, jiri VTM (mgbasa ozi na -ebugharị nje) nke nwere mgbakwunye antifungal na ọgwụ nje. Maka ọdịbendị nje ma ọ bụ fungal, bufee akọrọ ma ọ bụ na obere mmiri na -adịghị mma. Zere ntu oyi ugboro ugboro na ịchacha ihe nlele.
Ewezuga ihe nchịkọta akọwapụtara nke ọma na tebụl, na-emesi obi ike na akụrụngwa na akụrụngwa ndị ọzọ dị: dịka igbe mbupu na akpa nchịkọta na nkwakọ ngwaahịa, igwe nju oyi na ngwugwu oyi ma ọ bụ akpụrụ kpọrọ nkụ, akụrụngwa ịse ọbara na-adịghị mma (dịka agịga, sirinji na ọkpọ), akara na akara na -adịgide adịgide, PPE, ihe maka mmetọ ihu.
Maka nchọpụta nje, enwere ike iji nlele ọbara chọpụta nje site na usoro immunological, ebe a na -atụ aro RTqPCR ịchọpụta nCov nucleic acid maka nchọpụta ngwa ngwa na nke ziri ezi karị. Dị ka nje
Amaara usoro, ọdịnaya nke viral nucleic acid dị na nlele nwere ike chọpụta ngwa ngwa naanị
ịhọrọ reagents kwesịrị ekwesị na ihe nrịbama dabara
(Nchọpụta nchọpụta nke Wuhan coronavirus 2019 site na RTPCR, Protocol na ntule izizi dị ka nke Jenụwarị 13, 2020) Dị ka onye ndu n'ọhịa mmịpụta na ịchọpụta nucleic acid na China, TIANGEN BIOTECH (BEIJING) CO., LTD. enyela nje aka-ụkwụ-ọnụ na ihe nrịba ama nke nje virus A (H1N1) maka ihe karịrị nde mmadụ iri. Na 2019, etinyere nje nje nucleic acid nke nje TIANGEN na ihe nlele ihe ọkụkụ ezì 30 nke Africa, na -enye onyinye pụrụ iche na nchoputa na mgbochi nke ọrịa ezì oge gboo n'Afrịka na China. TIANGEN abụghị naanị na -enye mwepụta nje ngwa ngwa na nke ziri ezi na ihe nrụpụta ihe, kamakwa ọ na -enyekwa ngwa ngwa na -egweri anụ ahụ dabara adaba yana ndị na -ewepụta acid nucleic akpaka, nke mejupụtara usoro zuru oke maka mwepụ nje na nchọpụta.
Ngwọta Nucleic Acid na -akpaghị aka
TIANGEN Nucleic Acid Extractors akpaaka bụ nyiwe akpaka maka mmịpụta acid nucleic site na iji usoro bead magnetik. Ngwa nke ikpo okwu ndị a abụghị naanị na -ebelata oke ọrụ nyocha na ngalaba iche, kamakwa ọ na -ebelata njehie arụmọrụ nke mmịpụta akwụkwọ ntuziaka, wee hụ na ịdị n'otu na nkwụsi ike nke ịdị mma nke nucleic acid ewepụtara.
TIANGEN Automatic Nucleic Acid Extractor nwere ntinye dị iche iche (gụnyere ọwa 16, 24, 32, 48, 96), enwere ike iji reagents kwekọrọ maka mwepụ nucleic acid site n'ụdị ụdị nlele dị iche iche. TIANGEN na -enyekwa nrụpụta reagent ahaziri iche yana ọrụ ntinye ngwa dịka ihe nnwale dị iche iche si dị.
TGrinder H24 Homogenizer anụ ahụ
● A na -ejikarị ya eme ihe na -egweri ma na -ekekọta anụ ahụ dị iche iche na nsị nsị
Site n'iji ikike homogenization ugboro 2-5 karịa nke ngwa ọdịnala
● Ịkwa akwa na ịkpakọ ọnụ, na -ezere mmetọ obe
Ngwa nchedo akpaaka iji kpuchido nchekwa nke ndị ọrụ ụlọ nyocha
TGuide S32 Automatic Nucleic Acid Extractor
Nlele nlele: 1-32 samples
Olu nhazi: 20-1000 μl
Type Ụdị nlele: Ọbara, selụ, anụ ahụ, nsị, nje na nlele ndị ọzọ
Oge nhazi: Ruo nkeji 8 iji nweta nje nucleic acid
Mode Ọnọdụ njikwa: ọnọdụ njikwa abụọ nke Windows Pad na bọtịnụ ihuenyo
Development Mmepe nke ikpo okwu: Mepere emepe, nweere onwe ya dakọtara na reagents
● TGuide S32 Magnetik Viral DNA/Kit RNA (DP604)
Application Ngwa sara mbara: DNA/RNA nwere nje dị elu nwere ike mee ka ọ dị ọcha site n'ọbara, plasma, nlele swab, usoro ọgwụgwọ anụ ahụ yana usoro ichekwa nje dị iche iche.
● Dị mfe ma rụọ ọrụ nke ọma: Ngwaahịa a dabara nke ọma na TGuide S32 Automatic Nucleic Acid Extractor, nke nwere ike wepụta nje DNA/RNA na mkpụrụ dị elu, ịdị ọcha dị elu, ịdị mma na ntụkwasị obi.
Applications Ngwa ala ala: Acid nucleic acid dị ọcha dabara maka nnwale nnwale nke nje PCR na PCR ozugbo.
Ngwọta Nlekọta Acid Nucleic Acid
Dị ka onye ndu n'ọhịa mmịpụta na ịchọpụta nucleic acid, TIANGEN nwere usoro nchịkọta ngwaahịa nucleic acid kachasị na China, nke dị mma maka nchọpụta coronavirus site n'ụdị nlele niile: ọbara, ọbara/plasma, anụ ahụ, swab, nje , wdg.
TIANamp Nje Virus DNA/RNA Kit (DP315)
Efficiency arụmọrụ dị elu: Enwere ike nweta DNA na RNA nje dị elu site na nsacha ngwa ngwa yana nkwughachi dị elu.
Urity Ịdị ọcha dị elu: Mwepụ kpamkpam nke ihe na -emerụ emerụ na ihe mgbochi maka ngwa mgbada.
Safety Nchekwa dị elu: Mwepụ ihe nrụpụta ihe ma ọ bụ ọdịda ethanol adịghị mkpa.
Ngwọta Nchọpụta Nje Virus
1. Sistemụ Pipetting Automatic TEasy
Accuracy Izi ezi dị elu: Ihe mgbochi jụrụ oyi nwere ike idobe okpomọkụ nke ihe nlele reagent n'okpuru 7 ℃ maka ihe karịrị 60 min. A na -ahazi APM ọ bụla dịka ụkpụrụ mba ụwa siri dị, nke nwere nkenke dị elu karịa pipetting akwụkwọ ntuziaka.
Operation Ọrụ dị mfe: Obere nha. Ibu arọ. Enweghị ngwá ọrụ ọ bụla maka ndị na -anọchi block. Usoro nkwadebe PCR/qPCR wuru. Ọrụ ntuziaka dị mfe nke nkwadebe ngwọta PCR.
Application Ngwa sara mbara: Enwere ike iji ya maka pipetting efere efere 96/384, PCR, qPCR, nchọpụta mkpụrụ ndụ na nnwale ndị ọzọ dị elu.
FastKing Otu Nzọụkwụ RT-qPCR Kit (Nyocha) (FP314)
FastKing Otu Nzọụkwụ RT-qPCR Kit (Probe) (FP314) nke TIANGEN mepụtara bụ ngwa nchọta ntụgharị nke ọnụnọ nke ọnụnọ nke ukwuu dabere na usoro nyocha, nke emebere maka nchọpụta mkpụrụ ndụ ihe nketa na nlele dị iche iche. KingRTase dị na kit bụ transcriptase ọhụrụ gbanwere agbanwe, nke nwere mmekọrịta RNA siri ike na nkwụsi ike ọkụ, yana arụmọrụ ntụgharị ntụgharị ka mma yana ike ịgbatị nke ndebiri RNA dị mgbagwoju anya. A na -etinyekwa mmalite mmalite ọhụrụ Taq DNA polymerase iji nye mmeghachi omume PCR arụmọrụ dị elu na nkọwapụta. Na mgbakwunye, ngwa ahụ na-eme ka ihe ndị ahụ dị mfe nke ukwuu site na ịgwakọta Taq na MLV enzyme n'ime ngwakọta enzyme, yana ịgwakọta ihe ntinye ion, dNTPs, PCR stabilizer na nkwalite n'ime MasterMix, ka usoro ngwakọta ọtụtụ ihe wee bụrụ.
dị mfe.
Efficiency arụmọrụ dị elu: ezigbo transcriptase na DNA polymerase na -eme ka mmeghachi omume dị elu
● Nweghachị dị mma: Polymerase nwere ike ịgụ site na ndebiri RNA nwere ọdịnaya GC dị elu yana nhazi nke abụọ dị mgbagwoju anya
Applications Ngwa sara mbara: Ngwa dị elu maka ndebiri RNA nwere adịghị ọcha sitere n'ụdị dị iche iche
Mmetụta dị elu: Enwere ike ịkọwapụta ndebiri dị ala dịka 1 ng, ọkachasị maka ndebiri n'ụba
Ihe Nlereanya maka Nchọpụta Nje Virus
TGuide S32 Magnetic Viral DNA/RNA Kit (DP604) wepụtara nucleic acid nke H5 avian influenza. Ejiri FastKing Otu Nzọụkwụ RT-qPCR Kit (nyocha) (FP314) maka nchọpụta RT-qPCR site na iji akara H5 avian influenza primers na nyocha.
Ejiri ABi7500Fast mee nchọpụta RT-qPCR. Nsonaazụ H5 avian influenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6 na 10-7 dilution) sitere n'omume 200 showl na-egosi mkpụrụ mmịpụta nucleic acid dị elu, nke nwere ike izute ụdi na-esote. mkpa nke ntụgharị ntụgharị, PCR, RT-PCR, PCR ozugbo, wdg PCR na-ewepụta nsonaazụ ya na oke mmetụta, nzaghachi dị mma yana ahịrị njupụta dị nro. Enwere ike ịchọpụta ọkwa dị iche iche nke nlele nke nje nucleic acid n'ụzọ ziri ezi.
Oge ezipụ: Apr-11-2021
