Protection Nchedo nke ọma: Chebe RNA n'ọbara ọhụrụ ka ọ ghara ịla n'iyi.
■ Ọ dị mma: Ịwa ahụ na -ewe naanị nzọ ụkwụ 2 ma dabara adaba maka ịchekwa nnukwu nlele ọbara.
Compat Ndakọrịta zuru oke: Kwekọrọ na TIANGEN Silicon Matrix Membrane Purple Kit iji wepụta RNA ọbara ma ọ bụ DNA iji hụ na mmịpụta mkpụrụ na ịdị ọcha.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
Ihe: 100 μl ọbara òké ọhụrụ Usoro: Mkpokọta RNA nke ọbara òké ọhụrụ (echekwara na RNALock Reagent) nke echekwara na 15-25 ℃, 4-8 ℃, -20/-70 ℃ dịpụrụ adịpụ site na iji RNAprep Pure Blood Kit (4992238) na-eso usoro RNALock. . Nsonaazụ: Biko lee foto agarose gel electrophoresis dị n'elu. Ejiri 4-6 μl nke 50 μl eluates kwa otu ụzọ. M: TIANGEN DNA Marker III; Lane 1-2 (njikwa dị mma): RNA dị ọcha site n'ọbara ọhụrụ; Lane 3-4: A na-asachapụ RNA site na nlele ọbara echekwara na 15-25 ℃ maka ụbọchị abụọ. Lane 5-6: RNA dị ọcha site na nlele ọbara echekwara na 4-8 ℃ maka otu izu. Lane 7-8: A na -asachapụ RNA site na nlele ọbara echekwara na -20 ℃ ma ọ bụ -70 ℃ maka ọkara afọ. A na -eme electrophoresis na 6 V/cm maka nkeji 30 na agarose 1%. |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..