SuperReal PreMix Color (SYBR Green) Featured Image

Agba SuperReal PreMix (SYBR Green)

Nchebara echiche na kpọmkwem ihe e ji esiji ákwà fluorescence quantitative reagent.

Ngwaahịa a bụ reagent pụrụ iche maka PCR ozugbo na-eji SYBR Green I chimeric fluorescence. Ngwaahịa a bụ preMix ịta 2 mixed agwakọtara yana ezigbo mkpokọta nke SYBR Green I chọrọ maka mmeghachi omume PCR ozugbo. A na -agbakwunye ihe ngosi ahụ na ngwaahịa a, nke bara uru maka itinye ọtụtụ nlele ma na -ebelata ohere nke misoperation.

SuperReal Agba PreMix na -anabata ihe pụrụ iche na -amalite mmalite DNA polymerase (HotStart gbanwere kemịkalụ). Taq DNA polymerase na mgbochi mgbochi gbanwere Anti Taq DNA polymerase), a na -ejikwa arụmọrụ nkwalite nkwalite dị elu, nkọwapụta njupụta dị elu yana oke ntụkwasị obi yana yana sistemụ nchekwa nke ọma. Ihe reagent a kwalitere nwere uru ndị a ma ama na nkọwapụta nkwalite mgbe emezigharị ihe.

Nwamba. Mba	Nkwakọ ngwaahịa
4992777	20 µl × 125 rxn
4992876	20 µl × 500 rxn
4992902	20 µl × 5000 rxn

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Nkọwa ngwaahịa

Nlereanya Nnwale

Mkpado ngwaahịa

Atụmatụ

■ A na -agbakwunye ihe ngosi agba na reagents iji zere njehie mgbakwunye mgbakwunye ma ọ bụ nhapụ n'ihi ọnụ ọgụgụ dị ukwuu.
Nnukwu ọnụ ahịa fluorescence, mmetụta dị elu na nkọwapụta dị elu.
Mgbe achọpụtachara nke ọma, ihe ngosi agaghị emetụta uru fluorescence na nsonaazụ ọnụọgụ nke agba.
Kwesịrị ekwesị maka ọtụtụ ngwa PCR nkịtị (Roche, Bio-rad, ABI, wdg).

SuperReal PreMix Color (SYBR Green）

Ngwa

Ọ dabara maka ụdị nnwale dị iche iche dịka nyocha okwu na nchọpụta acid nucleic site na SYBR Green usoro dị iche iche ozugbo PCR. Ọ dabara nke ọma maka nnwale nnwale dị elu yana ọnụ ọgụgụ dị ukwuu nke ihe nlele yana arụ ọrụ ntinye ihe mgbagwoju anya.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: Tiantough Genotyping qPCR PreMix (nyocha)

Osote: Agba SuperReal PreMix (nyocha)

Ọgụgụ. 1. Eserese nke oghere cation amplifi na agbaze agbazere enwetara site na ịtụle mkpụrụ ndụ HS1 mmadụ site na iji TIANGEN SuperReal PreMix Plus (SYBR Green) (A) na Agba SuperReal PreMix (SYBR Green) (B). A na -atụgharị ndebiri ahụ maka gradient ịta ahụhụ 5. Ụkpụrụ Ct (C) enwetara site na ntụnyere na -egosi na mmụba nke ngwa abụọ ahụ nwere nnukwu uche, nkọwapụta na nkwụsi ike. Emere nnwale ahụ na Roche LightCycler480.

Onyonyo 2. Eserese mgbatịkwu na agbaze agbazere enwetara site na nha nha mkpụrụ ndụ HS5 nke mmadụ site na iji TIANGEN SuperReal PreMix Agba (SYBR Green) yana ngwaahịa dị mkpa sitere na onye na -eweta T (A: TIANGEN, B: onye na -ebubata T. Gbasaa template maka gradients 5, ma tulee ụkpụrụ Ct (C). Nsonaazụ na-egosi na SuperReal PreMix Agba (SYBR Green) nwere ikike dị elu, nke akọwapụtara nke ọma na nkwụsi ike. Nnwale ahụ mere na Bio-Rad CFX96.

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