Talent qPCR PreMix (SYBR Green) Featured Image

Onyinye qPCR PreMix (SYBR Green)

Ezi iguzogide nnyonye anya adịghị ọcha na ngụkọ ngwa ngwa nke ndebiri dị mgbagwoju anya.

Ngwaahịa a bụ reagent pụrụ iche maka Real-Time PCR n'iji SYBR Green I chimeric fluorescence. 2 × Talent qPCR PreMix na-anabata Anti Taq DNA polymerase, yana sistemụ qPCR pụrụ iche nwere ike hụ na ngwaahịa ahụ mere mmeghachi omume qPCR ngwa ngwa na ngwa PCR niile. Enwere ike belata oge mmeghachi omume qPCR site na 50%. Na mgbakwunye, mgbakwunye nke ihe H-Competitor na akụkụ EP na Buffer na-eme ka ngwaahịa ahụ nwee ọtụtụ ụdị nlele ụwa niile. Ọ nwere arụmọrụ dị mma maka ndebiri nwere usoro dị elu dị mgbagwoju anya, ndebiri nwere ihe ndị ọzọ na -egbochi PCR, nkwalite ogologo mkpirisi, wdg.
Ngwaahịa ahụ nwekwara njiri mara ọnụego mmụba dị elu, nkọwapụta njupụta dị elu, oke ekwenye ekwenye, wdg, yana enwere ike nweta nsonaazụ ngwa ngwa na -enweghị mmetụta PCR.

Nwamba. Mba	Nkwakọ ngwaahịa
4992882	20 µl × 125 rxn
4992775	20 µl × 500 rxn
4992866	20 µl × 5000 rxn

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Nkọwa ngwaahịa

Nlereanya Nnwale

Mkpado ngwaahịa

Atụmatụ

Poly Polymerase a na -emegide nke ukwuu nwere ike belata oge mmeghachi omume site na ọkara.
H-Competitor na-asọ mpi na njikọ hydrogen, nke na-ahụ maka nrụpụta nkwalite dị elu nke ndebiri nwere ọdịnaya GC dị elu, usoro nke abụọ dị mgbagwoju anya na ndebiri ogologo.
Akụkụ EP na -eme ka usoro PCR kwụsie ike, na -echebe ọrụ enzyme nke ọma ma na -egbochi nnyonye anya nke ihe mgbochi dị iche iche.
Tube tube transperent na -enweghị agba na -anọchi tube aja aja, nke mere na a ga -ahụ ego fọdụrụnụ.

Ngwa

Ọ dabara maka nyocha okwu, nchọpụta nucleic acid na ụdị nnwale ndị ọzọ na ngwa PCR dị iche iche site na usoro SYBR Green, ọkachasị maka GC dị elu, usoro nke abụọ dị mgbagwoju anya, ihe fọdụrụ adịghị ọcha na nha ogologo.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: FastFire qPCR PreMix (nyocha)

Osote: Ngwa nyocha HRM (EvaGreen)

Experimental Example

Mkpụrụ ndụ UH11 mmadụ (GC: 73.4%) bụ nke TIANGEN Talent qPCR PreMix (n'aka ekpe) na ngwaahịa dị mkpa sitere n'aka onye na -ebunye A (n'etiti) na B (n'aka nri). Nsonaazụ gosiri na e jiri ya tụnyere ndị na -ebubata ihe ndị ọzọ, Talent qPCR PreMix nwere akụkụ ọnụọgụ doro anya maka gradients 5, ọnụ ahịa Ct dị ala, ọnụ ahịa fluorescence dị elu, na ọnụ ụzọ na -agbaze otu, na -egosi Talent qPCR PreMix nwere ezigbo mmegharị na arụmọrụ nkwalite na ndebiri GC dị elu.

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