■ Ọ dị mfe iji: Enwere ike ijikwa ọrụ ngwanrọ n'ime 1 h. Mmemme nkwadebe PCR/qPCR wuru nwere ike gbanwee ma bufee ya ngwa ngwa.
Ables Ngwa oriri dakọtara: ọnụ ọkpọ pipette na -agbanwe agbanwe na sistemụ Beckman Biomek 3000.
Maintenance Ndozi dị mfe: Enwere ike dochie modulu pipetting akpaaka (APM) ngwa ngwa wee zighachi ya maka nbipu.
■ Ezigbo oke na nkenke.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
Ọgụgụ 1: nsonaazụ nsonaazụ usoro qPCR na -egosi mmegharị nke ọma Ihe atụ cDNA nke mkpụrụ ndụ 7 μl NIH 3T3 na-agwakọta ugboro 4 na mmiri 21 μl na nha 1: 4. Roche LightCycler 480 ezigbo PCR ngwa ngwa na SuperReal PreMix Plus (SYBR Green) maka nchọpụta. |
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Ọgụgụ 2: nkenke dị elu ma e jiri ya tụnyere pipetting akwụkwọ ntuziaka (N'aka ekpe: Akwụkwọ ntuziaka; Aka nri: TEasy Pipetting Automated) 4 kwughachiri nkwalite GAPDH mmadụ (usoro dị elu). Tinye 2 μl cDNA na 18 Masterl MasterMix iji mepụta usoro mmeghachi omume 20 μl. Ejiri Roche LightCycler 480 ezigbo ihe PCR na-ebu ọnụ fluorescence na SuperReal PreMix Plus (SYBR Green) maka nchọpụta. |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..