■ Ọ dị mfe yana ngwa ngwa: Enwere ike nweta DNA/RNA nke na -efe efe n'ime minit 40.
Rap Ultrapure: DNA/RNA malitere ịrịa nje nwere ịdị ọcha dị elu.
Nrụpụta dị elu: Enwere ike ị nweta 1-32 nlele na TGuide S32 Automatic Nucleic Acid Extractor.
Ụdị: Mwepụ ụdịdị ihe ndọta.
Ịlele: ọbara, plasma, lymph, mmiri ara na-enweghị sel, oke ọdịbendị sel, mmamịrị, ngwọta nchekwa nje, wdg.
Ebumnuche: DNA nje/RNA
Malite olu: 200 μl
Oge ọrụ: Nkeji 40
Ngwa ala ala: RT-PCR/RT-qPCR, owuwu ụlọ NGS, mmeghachi omume enzyme, wdg.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
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Ihe nlele: antigen ọkọlọtọ nke nje H5 nje Avian 10-2, 10-3, 10-4, 10-5, 10-6 na nke 10-7 ntughari gradient na njikwa NTC na RNA nje na-agbari na 100 μl RNase-Free ddH2O. Ngwa nchọpụta RT-qPCR: TIANGEN FP314, FastKing Otu Nzọụkwụ qRT-PCR kit (Nyocha) Ngwa RT-qPCR: AB 7500 Sistemụ PCR ngwa ngwa |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..