TGuide Nje Virus DNA/RNA Kit

Maka iwepụta DNA/RNA nje site n'ọbara, plasma, mmiri ahụ na-enweghị sel ma ọ bụ usoro nchekwa nje.

TGuide Virus DNA/RNA Kit bụ nke emebere maka ịwepụta nucleic acid site na nje site na iji TGuide Series Automatic Nucleic Acid Extractor. Ejila ihe eji eme plastik eji eme ihe iji wepu DNase/RNase, na nlele nke ọ bụla na -arụ ọrụ n'adabereghị. Usoro a nwere ike zere ohere dị iche iche nke mmetọ obe n'etiti nlele. Ngwa ahụ nwere ike wepụta nje DNA ma ọ bụ RNA site na nlele 200 μl /400 μl n'otu oge. Ọ bụ echekwa na adaba iji.

Nwamba. Mba	Nkwakọ ngwaahịa
Ahịa OSR-M202	48 ngụkọta ọnụ

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Nkọwa ngwaahịa

Nlereanya Nnwale

Ajụjụ

Mkpado ngwaahịa

Ngwa

Acid nucleic acid dị ọcha dabara maka PCR dị elu, RTPCR, PCR ọnụọgụ, RT-PCR nha na nnwale ndị ọzọ. Enyochala ngwa ahụ site na nchọpụta ala nke HBV, HCV, HIV na nje influenza.

Atụmatụ

Extra Mwepụta dị mfe na ngwa ngwa: Ngwa ngwa TGuide dabere n'ụkpụrụ nke ịdị ọcha acid nucleic site na beads magnet, na usoro mwepụ nke nucleic acid nwere ike mezue na nkeji 57.
Enweghị mmetọ: A na-ebuju katrij reagent akpọchiri akpọchi iji gbochie mmetọ.
Yield Mkpụrụ dị elu: Ngwa ahụ nwere Carrier RNA dị elu iji melite arụmọrụ nke njide acid nucleic.
■ Enweghị mmịpụta phenol na chloroform: Enweghị ihe mgbaze ihe ọkụkụ na -emerụ ahụ mmadụ dịka phenol na chloroform dị mkpa.
Sample Ntughari nlele nke mbụ: 200 μl/400 μl nlele nwere ike wepụta ya na ngwa ndị kwekọrọ.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: TGuide Plasma DNA Mgbapụta Ngwa (1.2ml)

Osote: TGuide Plant Genomic DNA Kit

Nchọpụta ọnụọgụ PCR nke fluorescence nke HBV

Ọgụgụ 1: sachapụ ọbara patientl dị mma 200 nke nwere HBV nke mkpokọta dị iche iche site na iji TGuide Virus DNA/RNA Kit. DNA nke nje HBV enwetara etisasịwo na nchekwa 60 μl elution.
Nchọpụta PCR nke Nest nke HCV

Ọgụgụ 2: Wepụ ihe nlele ọbara nwere ọnụ ọgụgụ dị iche iche nke nje HCV site na TGuide Virus DNA/RNA Kit, wee chọpụta nsonaazụ ya site na PCR akwụrụ.
1: 5 × 10⁰ HCV ọbara 2: 5 × 10¹ Ọbara HCV
3: 5 × 10² HCV Ọbara 4: 5 × 10³ Ọbara HCV
5: 5 × 10⁴ HCV Ọbara 6: 5 × 10⁵ Ọbara HCV
P: Njikwa dị mma N: Njikwa na -adịghị mma
M: 100 bp ọkwa DNA

Ajụjụ: Obere ma ọ bụ enweghị DNA dị elu.

A-2 Lysis ezughi oke nke nlele ahụ-Agaghị agwakọta ihe nlele ahụ nke ọma na ihe nchekwa lysis. A na-atụ aro ka agwakọta ya nke ọma site na pulse-vortexing maka ugboro 1-2. —Ndị lysis sel na -ezughị ezu sitere na mbelata ọrụ proteinase K. -Ihe ezughi oke cell lysis ma ọ bụ mbibi protein n'ihi ezughị oke ịsa ahụ ọkụ. A na -atụ aro ka ebipụ anụ ahụ n'ime obere iberibe ma gbatịkwuo oge ịsa ahụ iji wepu ihe niile fọdụrụ na lysate.

A-3 mgbasa ozi DNA ezughi oke. —Enweghị ethanol ma ọ bụ pasent dị ala karịa 100% ethanol tupu agbakwunye lysate na kọlụm ogho.

A-4 Uru pH nke ịkwa iko na-adị oke ala. Gbanwee pH n'etiti 8.0-8.3.

Ajụjụ: DNA anaghị eme nke ọma na nnwale mmeghachi omume enzymatic ala.

Ethanol fọdụrụnụ dị n'elu.

- Enwere ihe nchekwa ịsa ahụ PW dị elu. Enwere ike wepu ethanol site na ịgbanye kọlụm maka nkeji 3-5, wee tinye ya na ụlọ okpomọkụ ma ọ bụ incubator 50 for maka nkeji 1-2.

Ajụjụ: Mmebi DNA

A-1 Ihe nlele ahụ abụghị nke ọhụrụ. - Wepụta DNA nlele dị mma ka ọ bụrụ akara iji chọpụta ma DNA dị na nlele ahụ emebiela.

A-2 ọgwụgwọ tupu ọgwụgwọ adịghị mma. —N'ihi na ọ na -egwepịa mmiri nitrogen dị oke mmiri, iweghachite mmiri, ma ọ bụ oke nlele.

Ajụjụ: Etu ị ga -esi mee ọgwụgwọ maka mmịpụta gDNA?

Nhazi ọkwa kwesịrị ịdị iche iche maka nlele dị iche iche. Maka nlele osisi, gbaa mbọ hụ na igwu mmiri nitrogen dị mmiri mmiri. Maka nlele anụmanụ, homogenate ma ọ bụ gwuo nke ọma na nitrogen mmiri mmiri. Maka nlele nwere mgbidi selụ nke siri ike imebi, dịka nje bacteria G+ na yist, a na -atụ aro ka ị jiri lysozyme, lyticase ma ọ bụ ụzọ igwe iji mebie mgbidi sel.

Ajụjụ: Gịnị bụ ihe dị iche na ngwa mwepụta gDNA osisi atọ 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Osisi Genomic DNA Kit na-anabata usoro dabere na kọlụm nke chọrọ chloroform maka mmịpụta ya. Ọ dabara nke ọma maka nlele osisi dị iche iche, yana ntụ ntụ akọrọ. Ngwa Hi-DNAsecure Plant Kit bụkwa nke dabere na kọlụm, mana na-enweghị mkpa mmịpụta phenol/chloroform, na-eme ka ọ dị mma na anaghị egbu egbu. Ọ dabara maka osisi nwere polysaccharides dị elu na ọdịnaya polyphenol. 4992709/4992710 DNAquick Plant System na-anabata usoro dabere na mmiri. Mwepu Phenol/chloroform adịghị mkpa. Usoro nsacha dị mfe na ngwa ngwa na -enweghị oke maka mbido mbido nlele, yabụ ndị ọrụ nwere ike ịhazigharị ego ahụ n'ụzọ dị mfe dịka ihe nnwale chọrọ si dị. Enwere ike nweta nnukwu iberibe gDNA nwere nnukwu mkpụrụ.

Kedu ihe amịpụtara mkpụrụ gDNA sitere na ntinye ọbara 1 ml site na TIANamp Blood DNA Kit?

Enwetara DNA nke mkpụrụ ndụ ihe nketa site na mpịakọta dị iche iche nke ọbara mmadụ site na TIANamp Blood DNA Kit. Nsonaazụ ya dị ka ndị a. Edepụtara nsonaazụ ya ka ọ bụrụ naanị ntụnyere, nsonaazụ mmịpụta n'ezie dabere na ọnọdụ nke nlele.

Ajụjụ: Enwere ike iji 4992207/4992208 na 4992722/4992723 wepụta DNA mkpụkọ ọbara?

Enwere ike ịme mwepụ DNA nke ọbara site na iji reagents enyere na ngwa abụọ a site na ịgbanwere usoro ahụ na ntuziaka akọwapụtara maka mmịpụta DNA ọbara. Enwere ike ịnye akwụkwọ dị nro nke usoro mmịpụta DNA na -egbochi ọbara mgbe ị rịọrọ.

Ajụjụ: Mgbe ị na -etinye TIANamp Genomic DNA Kit, kedu ka esi agbaji anụ ahụ ọhụrụ ka ọ bụrụ nkwụsị sel?

Kwụsị ihe nlele ọhụrụ na 1 ml PBS, saline nkịtị ma ọ bụ nchekwa TE. Kpochapụrụ ihe nlele ahụ nke ọma site na homogenizer ma na -anakọta mkpali na ala nke tube site na centrifuging. Wepu ihe dị elu, wee jiri mbughari 200 GAl ga -ebughachi mmiri ozuzo. Enwere ike ịme nsacha DNA na -esote dịka ntuziaka ahụ si dị.

Ajụjụ: Kedu ka esi họrọ ngwaahịa maka mmịpụta DNA site na plasma, ọbara na ihe nlebara ahụ?

Maka ịdị ọcha nke gDNA na plasma, ọbara na ihe nlebara ahụ, a na -atụ aro TIANamp Micro DNA Kit. Maka ịdị ọcha nke nje gDNA site na nlele ọbara/plasma, a na -atụ aro TIANamp Virus DNA/RNA Kit. Maka ịdị ọcha nke gDNA nje site n'ọbara na nlele plasma, a na -atụ aro TIANamp Bacteria DNA Kit (ekwesịrị itinye lysozyme maka nje dị mma). Maka nlele mmiri, a na-atụ aro Hi-Swab DNA Kit na TIANamp Bacteria DNA Kit.

Ajụjụ: Etu ị ga -esi họrọ akụrụngwa maka mmịpụta gDNA site n'omume fungi?

A na -atụ aro Kit Kit DNAsecure ma ọ bụ Sistemụ Pụkụkụ DNA maka iwepụta mkpụrụ ndụ ihe nketa fungal. Maka mmịpụta mkpụrụ ndụ ihe nketa yist, a na-atụ aro Kit Kit DNA Yist (lyticase kwadebere n'onwe ya).

Kit mwepụ Dna Cell na -akwụghị ụgwọ