TIANprep N96 Plasmid Kit

Nlekota oge nke ọnọdụ lysis yana mmịpụta dị elu nke plasmids dị ọcha

Akpụkpọ ahụ nke N96 Plate CP3 dị na ngwa a na -ewere ihe ọhụrụ silica matrix matriki nke TIANGEN mepụtara, nke nwere ike rụọ ọrụ nke ọma ma kpoo DNA plasmid nke ọma, wepu protein na adịghị ọcha ndị ọzọ ruo oke, wee si otú a na -ahụ ịdị ọcha nke plasmid ahụ ewepụtara. Ihe ruru 1.3 ml nke ọdịbendị nje plasmid dị elu nwere ike ịhazi ya nke ọma, site na ya enwere ike wepụta 5-10 μg nke plasmid DNA ngwa ngwa (mkpụrụ ya metụtara ihe ndị dị ka nọmba njupụta plasmid, ọnọdụ nje, wdg. ). Enwere ike iji Plasmid DNA nke ngwa ahụ na nnwale bayoloji molekụla dịka mgbaze nri enzyme, usoro, nyocha ọbá akwụkwọ, njikọ na mgbanwe.

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Atụmatụ

Speed Ọsọ iwepụ ngwa ngwa.
Ejiri reagent TIANRed pụrụ iche, ọ nwere ike mata nke ọma ogo lysis nke nlele na usoro mwepụ iji hụ na arụmọrụ dị elu yana ịdị ọcha dị elu nke DNA plasmid.
Urity Ịdị ọcha dị elu: Enwere ike iji DNA plasmid dị ọcha na nnwale nnwale.
■ Enwere ike iji kit a rụọ ọrụ na -arụpụta ihe na -arụ ọrụ nke ukwuu nucleic acid.

TIANRed Agba egosi

TIANRed Color indicator:

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: TIANamp FFPE DNA Kit

Osote: TIANamp Ọbara DNA Midi Kit

	Enwetara Plasmid DNA site na 1 ml nke broth Escherichia coli (TOP10) nke abalị (LB medium) na-eji TIANprep Highpure N96 Plasmid Kit, yana nkezi 5-6 μg nke pBS plasmid dị ọcha dị elu. Ebubere plasmid ahụ n'ime olulu mmiri ọ bụla nke jel agarose 1%, electrophoresis chọpụtakwara ya.
	Enwetara Plasmid DNA site na 1 ml nke broth Escherichia coli (TOP10) nke abalị (LB medium) na-eji TIANprep Highpure N96 Plasmid Kit, yana nkezi 5-6 μg nke pBS plasmid dị ọcha dị elu. Vector: pBS; Plasmid ụdị: TOP10. ABI sụgharịrị plasmid ahụ. PRISM 3730XL DNA na -eso ụzọ.