TIANprep Rapid N96 Plasmid Kit

Mpụta dị elu, iwepụ ngwa ngwa nke obere plasmid.

Ngwa a na -anabata usoro mmiri ozuzo isopropanol iji nweta DNA plasmid. Ngwa a na-anabata efere nzacha 96 nke ọma N na Buffer III, nke TIANGEN mepụtara n'ụzọ pụrụ iche, ma nwee ike wepụta DNA plasmid dị elu ngwa ngwa yana rụọ ọrụ nke ọma. Ọ dị mma maka nkwadebe ngwa ngwa nke plasmids dị elu. Enwere ike ịhazi 1.3 ml nke ụdị nje plasmid dị elu n'otu olulu mmiri, na-ewepụta ihe nlele 96 nke DNA plasmid ngwa ngwa. Oke kacha kwa otu olulu mmiri bụ 10-20 μg (mkpụrụ ya na-emetụta ihe dịka ọnụọgụ nnomi plasmid, ọnọdụ nje, wdg). Enwere ike iji Plasmid DNA nke ngwa ahụ na nnwale bayoloji molekụla dịka mgbaze nri enzyme, usoro, nyocha ọbá akwụkwọ, njikọ na mgbanwe.