Ngwa EndoFree Maxi Plasmid V2

Ọcha nke mkpụrụ ndụ ihe nsure ọkụ na-enweghị ngwụcha endotoxin DNA plasmid akọwapụtara maka mkpụrụ ndụ nwere mmetụta.

EndoFree Maxi Plasmid Kit V2 na -anabata teknụzụ mgbasa ozi silica pụrụ iche iji jikọta DNA plasmid nke ọma. Buffer ER pụrụ iche na Filtration CS1 nwere ike wepu adịghị ọcha dịka endotoxin na protein. Usoro mwepụ niile na -ewe naanị elekere 1.
Akwadoro ọdịbendị nje bacteria akwadoro: Maka plasmid dị elu, a na-atụ aro ọkara nje bacteria 100 ml, nke nwere ike ị nweta plasmid 500-1500 μg. Maka plasmid dị obere, 200 ml nke nje bacteria ka akwadoro iji nweta mkpụrụ ihe ruru 50-300 μg.

Nwamba. Mba	Nkwakọ ngwaahịa
4992438	10 nkwadebe

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Nkọwa ngwaahịa

Usoro ọrụ

Nlereanya Nnwale

Mkpado ngwaahịa

Atụmatụ

P Ịdị ọcha dị elu: A nabatara teknụzụ mmiri ozuzo pụrụ iche nke endotoxin iji wepu endotoxin kpọmkwem.
■ Ọ dị mfe ịrụ ọrụ: A na -eji teknụzụ kọlụm adsorb kpọmkwem DNA plasmid, nke na -eme ka ọrụ ahụ dị mfe.
Trans Mgbanwe dị elu nke ọma: Kwesịrị ekwesị maka ntụgharị nke ọtụtụ ahịrị sel gụnyere sel nwere mmetụta endotoxin.
Applications Ngwa dịgasị iche iche: Enwere ike itinye plasmid ahụ dị ọcha na ntụgharị mkpụrụ ndụ anụmanụ na osisi yana nnwale nke bayoloji molekul.

Ngwa

Enwere ike iji DNA plasmid wepụtara site na iji ngwa a maka ọrụ dị iche iche oge niile, gụnyere mgbaze nri enzyme, PCR, usoro, ligation, mgbanwe na ntụgharị mkpụrụ ndụ dị iche iche.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: TIANprep N96 Plasmid Magnetik

Osote: Ngwa STIANqucik N96

Ihe nrụpụta ihe pụrụ iche nke endotoxin Buffer ER nwere ike wepu nsị endotoxin nke ọma na sistemụ mmeghachi omume, enwere ike nweta plasmid dị ọcha. Ihe fọdụrụ nke plasmid endotoxin bụ ≤ 0.1 EU/ml.

Eji EndoFree Maxi Plasmid Kit V2 mee ka plasmid ahụ dị ọcha na otu ngwaahịa sitere na Onye na -eweta 1 na onye na -ebunye ya abụọ na -ejikwa otu olu nke ịkwa akwa. Ejiri 1 ml plasmid kwa otu ụzọ iji tụọ mkpokọta nke plasmid. 100 ng nke plasmid nke spectrophotometer kpebisiri ike itinye ya n'otu gel iji chọpụta ma uru ịta ahụhụ dị elu.
Mmechi: Nsonaazụ electrophoresis na -egosi ntinye nke plasmid nke EndoFree Maxi Plasmid kit V2 wepụtara, ma ọ bụ nke dị elu ma ọ bụ nke dị ala, dị elu nke ukwuu karịa nke ndị na -ebubata ya 1 na 2 wepụtara.

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