Ngwa STIANqucik N96

Nchacha dị elu nke iberibe DNA nke 100 bp-10 kb.

Ngwa a na -agwakọta iberibe DNA na azịza mmeghachi omume dịka mgbaze enzyme, PCR, na -esite na N96 Plate CB2. Ọ nwere ike wepu adịghị ọcha dị ka protein, ogige ogige, ion nnu inorganic, oligonucleotide primers, wdg. DNA ahụ dị ọcha dabara maka nnwale nke bayoloji dị ka mgbachi nri enzyme, usoro, nyocha ọbá akwụkwọ, njikọta na mgbanwe.

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Atụmatụ

Rate Ọnụ mgbake nke iberibe DNA 100 bp-10 kb nwere ike iru karịa 80%.
Ọnụ ego kacha adịnye na DNA n'otu olulu mmiri bụ 5 µg.
P Ịdị ọcha dị elu: Enwere ike iji DNA dị ọcha mee nnwale nnwale.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: Ngwa EndoFree Maxi Plasmid V2

Osote: Ngwa ime ọcha TIANgel

Ejiri mpe mpe mpe akwa TIANquick N96 sachara ihe dị 900 bp, 3500 bp na 4500 bp.
Ebufere 3 ofl nke 50 μl eluant na 1% gel agrose, na electrophoresis rụrụ na 6 V/cm maka nkeji 20.
1, 2 na 3: A na -eji DNA TIANGEN TIANquick N96 eme ka DNA dị ọcha;
4: Emechara ka DNA dị ọcha site na iji ngwaahịa dị mkpa sitere na Onye na -eweta ngwaahịa A.

Experimental Example:

Dee ozi gị ebe a ma zitere anyị ya

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Ngwa ime ọcha TIANgel

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nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..

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