Nsonaazụ ngwa ngwa na elu: 5-70 μg plasmid DNA nwere ike wepụta ya n'ihe dị ka elekere 1.
Ọdịdị dị elu: Sistemu mwepụ pụrụ iche nke endotoxin jikọtara ya na Spin Column CP4 nwere ike adsorb nucleic acid ma wepu endotoxin na ọkwa pere mpe.
Applications Ngwa dịgasị iche iche: Kwesịrị ekwesị maka nnwale ndị a na-eme kwa ụbọchị dị ka mmachi endonuclease mgbaze, PCR, usoro, ligation, mgbanwe, yana nnwale nnwale dị elu dị ka ọgwụgwọ mkpụrụ ndụ ihe nketa, microinjection sel, ịgbachi nkịtị mkpụrụ ndụ, transcription, wdg.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
Kwesịrị ekwesị maka mmịpụta nke plasmids dị elu/dị ala Onyonyo 1. Mwepụta DNA plasmid site na olu dị iche iche nke ọdịbendị nje site na iji EndoFree Mini Plasmid Kit II. Mpịakọta ịdọ aka ná ntị: 200 μl; Mpịakọta na-ebugo: 3 μl plasmid pBR322 dị ala, 2 μl plasmid pBS dị elu. A na -eme electrophoresis na 6 V/cm maka nkeji 30 na 1% agarose. Mgbanwe mkpụrụ ndụ site na plasmid dị ọcha |
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Onyonyo 2 E bufere plasmid pEGFP nke EndoFree Mini Plasmid Kit II mere n'ime sel 293T. Achọpụtara nkọwa nke GFP na 48 h post-transfection |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..