EndoFree Mini Plasmid Kit II

Maka ịdị ọcha nke DNA plasmid transfexin na-enweghị nnwere onwe.

EndoFree Mini Plasmid Kit II na -anabata teknụzụ mgbasa ozi silica pụrụ iche iji jikọta DNA plasmid nke ọma. Usoro mwepụ endotoxin pụrụ iche nwere ike wepu adịghị ọcha dịka endotoxin na protein. Usoro mwepụ ahụ dum na -ewe naanị elekere 1, nke dabara adaba yana ngwa ngwa. Nsonye mmụba nke plasmid metụtara ọnụọgụ oyiri plasmid, ụdị nje ndị ọbịa na ọnọdụ ọdịbendị. Enwere ike wepụta ihe ruru 70 μg nke DNA plasmid site na 5-15 ml nke usoro ọdịbendị nje na-eri nri abalị.

Nwamba. Mba Nkwakọ ngwaahịa
4992422 50 ngụkọta ọnụ

Nkọwa ngwaahịa

Nlereanya Nnwale

Mkpado ngwaahịa

Atụmatụ

Nsonaazụ ngwa ngwa na elu: 5-70 μg plasmid DNA nwere ike wepụta ya n'ihe dị ka elekere 1.
Ọdịdị dị elu: Sistemu mwepụ pụrụ iche nke endotoxin jikọtara ya na Spin Column CP4 nwere ike adsorb nucleic acid ma wepu endotoxin na ọkwa pere mpe.
Applications Ngwa dịgasị iche iche: Kwesịrị ekwesị maka nnwale ndị a na-eme kwa ụbọchị dị ka mmachi endonuclease mgbaze, PCR, usoro, ligation, mgbanwe, yana nnwale nnwale dị elu dị ka ọgwụgwọ mkpụrụ ndụ ihe nketa, microinjection sel, ịgbachi nkịtị mkpụrụ ndụ, transcription, wdg.

Mmụba Plasmid

Plasmid Yield

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)


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    Experimental Example Kwesịrị ekwesị maka mmịpụta nke plasmids dị elu/dị ala
    Onyonyo 1. Mwepụta DNA plasmid site na olu dị iche iche nke ọdịbendị nje site na iji EndoFree Mini Plasmid Kit II.
    Mpịakọta ịdọ aka ná ntị: 200 μl; Mpịakọta na-ebugo: 3 μl plasmid pBR322 dị ala, 2 μl plasmid pBS dị elu.
    A na -eme electrophoresis na 6 V/cm maka nkeji 30 na 1% agarose.
    Mgbanwe mkpụrụ ndụ site na plasmid dị ọcha
    Experimental Example Onyonyo 2 E bufere plasmid pEGFP nke EndoFree Mini Plasmid Kit II mere n'ime sel 293T. Achọpụtara nkọwa nke GFP na 48 h post-transfection
    Dee ozi gị ebe a ma zitere anyị ya