Ala Magnetik na Kit DNA
Atụmatụ
Applic Ngwa dị ukwuu: Kwesịrị ekwesị maka iwepụta ụdị ihe nlele gburugburu ebe obibi dị ka ala akwa ifuru, ala ite ifuru, ala ubi, ala oke ọhịa, silt, ala uhie, ala ojii, uzuzu na ihe ndị ọzọ. Ọ dịkwa mma maka ịwepụta ihe nlele stool na obere eriri afọ.
Operation Ọrụ dị mma: Enwere ike imecha nnwale nnwale ahụ n'ime obere oge.
Urity Ịdị ọcha dị elu: Ejikọtara ya na ịdị ọcha bead magnet, DNA amịpụtara nwere ịdị ọcha dị elu ma enwere ike iji ya ozugbo na nnwale ala.
Ngwa
DNA nke e mere ka ọ dị ọcha nwere adịghị ọcha na ezigbo iguzosi ike n'ezi ihe, enwere ike iji ya ozugbo na nnwale ndị ọzọ dị n'okpuru ala nke bayoloji dị ka PCR, mgbaze enzyme, wdg.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
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Ewepụtara DNA Genomic site na 500 mg nke ubi ubi site na onye na -ama jijiji na igwe nri n'otu n'otu, na -eji TIANGEN Magnetic Soil And Stool DNA Kit na ngwaahịa dị mkpa sitere na onye na -ebunye M. 5 μl nke 100 μl eluate buuru 1% agarose gel electrophoresis. M: TIANGEN Onye kere D2000 |
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Ewepụtara DNA Genomic site na oche mmadụ na TIANGEN Magnetic Soil And Stool DNA Kit na ngwaahịa dị mkpa sitere na onye na -eweta M n'otu n'otu, PCR chọtara ya na nje bacteria 16S. Ejiri 5 μl nke ngwaahịa 20 μl PCR gaa na 1% electrophoresis gel agarose. M: TIANGEN Onye kere D2000 |
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Enwetara DNA nke anụ ahụ site na microorganisms eriri afọ site na iji TIANGEN Magnetic Soil And Stool DNA Kit na ngwaahịa dị mkpa sitere na Onye na -eweta M n'otu n'otu, PCR chọtara ya na ntụpọ izugbe 27F/1492R nke nje na ngwaahịa ihe dị ka 1500 bp. Ejiri 5 μl nke ngwaahịa 20 μl PCR gaa na 1% electrophoresis gel agarose. M: TIANGEN Onye kere D2000 |
A.
A-2 Lysis ezughi oke nke nlele ahụ-Agaghị agwakọta ihe nlele ahụ nke ọma na ihe nchekwa lysis. A na-atụ aro ka agwakọta ya nke ọma site na pulse-vortexing maka ugboro 1-2. —Ndị lysis sel na -ezughị ezu sitere na mbelata ọrụ proteinase K. -Ihe ezughi oke cell lysis ma ọ bụ mbibi protein n'ihi ezughị oke ịsa ahụ ọkụ. A na -atụ aro ka ebipụ anụ ahụ n'ime obere iberibe ma gbatịkwuo oge ịsa ahụ iji wepu ihe niile fọdụrụ na lysate.
A-3 mgbasa ozi DNA ezughi oke. —Enweghị ethanol ma ọ bụ pasent dị ala karịa 100% ethanol tupu agbakwunye lysate na kọlụm ogho.
A-4 Uru pH nke ịkwa iko na-adị oke ala. Gbanwee pH n'etiti 8.0-8.3.
Ethanol fọdụrụnụ dị n'elu.
- Enwere ihe nchekwa ịsa ahụ PW dị elu. Enwere ike wepu ethanol site na ịgbanye kọlụm maka nkeji 3-5, wee tinye ya na ụlọ okpomọkụ ma ọ bụ incubator 50 for maka nkeji 1-2.
A-1 Ihe nlele ahụ abụghị nke ọhụrụ. - Wepụta DNA nlele dị mma ka ọ bụrụ akara iji chọpụta ma DNA dị na nlele ahụ emebiela.
A-2 ọgwụgwọ tupu ọgwụgwọ adịghị mma. —N'ihi na ọ na -egwepịa mmiri nitrogen dị oke mmiri, iweghachite mmiri, ma ọ bụ oke nlele.
Nhazi ọkwa kwesịrị ịdị iche iche maka nlele dị iche iche. Maka nlele osisi, gbaa mbọ hụ na igwu mmiri nitrogen dị mmiri mmiri. Maka nlele anụmanụ, homogenate ma ọ bụ gwuo nke ọma na nitrogen mmiri mmiri. Maka nlele nwere mgbidi selụ nke siri ike imebi, dịka nje bacteria G+ na yist, a na -atụ aro ka ị jiri lysozyme, lyticase ma ọ bụ ụzọ igwe iji mebie mgbidi sel.
4992201/4992202 Osisi Genomic DNA Kit na-anabata usoro dabere na kọlụm nke chọrọ chloroform maka mmịpụta ya. Ọ dabara nke ọma maka nlele osisi dị iche iche, yana ntụ ntụ akọrọ. Ngwa Hi-DNAsecure Plant Kit bụkwa nke dabere na kọlụm, mana na-enweghị mkpa mmịpụta phenol/chloroform, na-eme ka ọ dị mma na anaghị egbu egbu. Ọ dabara maka osisi nwere polysaccharides dị elu na ọdịnaya polyphenol. 4992709/4992710 DNAquick Plant System na-anabata usoro dabere na mmiri. Mwepu Phenol/chloroform adịghị mkpa. Usoro nsacha dị mfe na ngwa ngwa na -enweghị oke maka mbido mbido nlele, yabụ ndị ọrụ nwere ike ịhazigharị ego ahụ n'ụzọ dị mfe dịka ihe nnwale chọrọ si dị. Enwere ike nweta nnukwu iberibe gDNA nwere nnukwu mkpụrụ.
Enwetara DNA nke mkpụrụ ndụ ihe nketa site na mpịakọta dị iche iche nke ọbara mmadụ site na TIANamp Blood DNA Kit. Nsonaazụ ya dị ka ndị a. Edepụtara nsonaazụ ya ka ọ bụrụ naanị ntụnyere, nsonaazụ mmịpụta n'ezie dabere na ọnọdụ nke nlele.
Enwere ike ịme mwepụ DNA nke ọbara site na iji reagents enyere na ngwa abụọ a site na ịgbanwere usoro ahụ na ntuziaka akọwapụtara maka mmịpụta DNA ọbara. Enwere ike ịnye akwụkwọ dị nro nke usoro mmịpụta DNA na -egbochi ọbara mgbe ị rịọrọ.
Kwụsị ihe nlele ọhụrụ na 1 ml PBS, saline nkịtị ma ọ bụ nchekwa TE. Kpochapụrụ ihe nlele ahụ nke ọma site na homogenizer ma na -anakọta mkpali na ala nke tube site na centrifuging. Wepu ihe dị elu, wee jiri mbughari 200 GAl ga -ebughachi mmiri ozuzo. Enwere ike ịme nsacha DNA na -esote dịka ntuziaka ahụ si dị.
Maka ịdị ọcha nke gDNA na plasma, ọbara na ihe nlebara ahụ, a na -atụ aro TIANamp Micro DNA Kit. Maka ịdị ọcha nke nje gDNA site na nlele ọbara/plasma, a na -atụ aro TIANamp Virus DNA/RNA Kit. Maka ịdị ọcha nke gDNA nje site n'ọbara na nlele plasma, a na -atụ aro TIANamp Bacteria DNA Kit (ekwesịrị itinye lysozyme maka nje dị mma). Maka nlele mmiri, a na-atụ aro Hi-Swab DNA Kit na TIANamp Bacteria DNA Kit.
A na -atụ aro Kit Kit DNAsecure ma ọ bụ Sistemụ Pụkụkụ DNA maka iwepụta mkpụrụ ndụ ihe nketa fungal. Maka mmịpụta mkpụrụ ndụ ihe nketa yist, a na-atụ aro Kit Kit DNA Yist (lyticase kwadebere n'onwe ya).
Ngwaahịa ngwaahịa
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