TIANprep Rapid Mini Plasmid Kit Featured Image

Ngwa TIANprep Rapid Mini Plasmid

Maka nsacha ngwa ngwa nke DNA plasmid nke ọkwa bayoloji molekụla site na usoro lysis alkaline.

TIANprep Rapid Mini Plasmid Kit na-kachasị site na teknụzụ alkaline lysis ọdịnala, nke enwere ike ịsacha DNA plasmid dị elu n'ime nkeji asatọ. Ihe nchekwa lysis ọhụrụ na -enye ohere mgbasa ozi DNA na akpụkpọ ahụ silica n'ihu nnukwu nnu. Ihe eji eme akpụkpọ ahụ silica bụ ihe pụrụ iche, na -arụ ọrụ nke ọma yana nke akọwapụtara nke ọma. Emebere ngwa a maka ịdị ọcha ruru 24 μg DNA plasmid site na 1-4 ml omenala nke abalịE.coli. Enwere ike itinye DNA plasmid nke ngwa a mere ka ọ dị ọcha na nnwale dị iche iche oge niile, dị ka mgbaze enzyme, PCR, usoro, ligation, mgbanwe, nyocha ọbá akwụkwọ, in vitro transcription na ntụgharị mkpụrụ ndụ sub-ọdịbendị nkịtị, wdg.

Nwamba. Mba	Nkwakọ ngwaahịa
4992191	50 ngụkọta ọnụ
4992192	200 ngụkọta

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Nkọwa ngwaahịa

Usoro ọrụ

Nlereanya Nnwale

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Atụmatụ

■ Ọsọ: Usoro dị mfe yana obere usoro iji mee ka DNA plasmid dị ọcha dị ọcha n'ime naanị nkeji 8.
Efficiency arụmọrụ dị elu: nwere ike sachapụ ihe karịrị 85% plasmid DNA site na E.coli.
■ E nyere ihe ngosi agba TIANRed ka ọ jikwaa ma chetara usoro niile n'oge nnwale iji hụ na ịdị ọcha nke plasmid dị elu.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: TGuide S32 Automatic Nucleic Acid Extractor

Osote: Ngwa Plasmid EndoFree Midi

Site na ọgwụgwọ lysate dị elu yana sistemụ nchekwa kachasị, TIANprep Rapid Mini Plasmid Kit nwere ike nweta ngwaahịa dị elu karịa ka ọ na-azọpụta ọtụtụ oge ọrụ.

Ntụnyere n'etiti TIANprep Rapid Mini Plasmid Kit na Supplier A (ngwongwo ịdị ọcha DNA nke plasmid) site n'ịsacha otu plasmid n'otu oge.

Mpịakọta nlele: 3 ml omenala E.coli n'otu ntabi anya (OD600 = 1.8); Olu olu: 50 μl; Oke mkpọtụ: 1 μl pBS, 3 pl pET30a, 3 μl pBR322.

A na -eme electrophoresis na 6 V/cm maka nkeji 30 na 1% jel agarose. M: TIANGEN DNA Marker IV.

Mmechi: Agarose gel electrophoresis na -egosi na TIANprep Rapid Mini Plasmid Kit nwere ike nweta otu mkpụrụ nke plasmid DNA dị ka nke ahụ site na ngwa mkpocha plasmid n'ozuzu n'ime nkeji 8.

TIANprep Rapid Mini Plasmid Kit