Nsonaazụ ngwa ngwa na nke dị elu: mkpụrụ ndụ plasma plasmid 200 μg-1.5 mg na-enye n'ime elekere 1 nwere oke nke nnukwu ihe mkpuchi.
Ịdị ọcha dị elu: A na-enweta DNA plasmid dị oke ọcha site na sistemụ nchekwa pụrụ iche na Spin Column CP6.
Efficiency Ọmarịcha arụmọrụ transfection: dabara maka nnwale nnwale nke ọtụtụ ahịrị sel.
Applications Ngwa dịgasị iche iche: Kwesịrị ekwesị maka mgbochi mgbaze enzyme, mgbanwe, usoro, microinjection, ịgbachi nkịtị na nnwale nnwale.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
Mwepụta plasmid dị elu/dị ala PBR322 plasmid dị obere, ewepụtara site na omenala nje 200 ml site na iji EndoFree Maxi Plasmid Kit, ka etinyere na 1 ml Buffer TB, yana mkpokọta 0.6 μg/μl. PBS plasmid dị elu, nke ewepụtara site na ọdịbendị nje 100 ml site na iji EndoFree Maxi Plasmid Kit, ka etolitere na 1 ml Buffer TB, yana mkpokọta 1.2 μg/μl. MIV: TIANGEN DNA Marker IV PBR322: 2 μl, pBS: 2 μl |
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pEGFP nwetara na EndoFree Maxi Plasmid Kit ka etinyere iche iche n'ime ahịrị mkpụrụ ndụ endotoxin 293T na sel Huh7 nwere mmetụta endotoxin site na TIANGEN TIANfect. Achọpụtara nkọwapụta nke GFP n'ime awa 24 ka emechara. |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..