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Ngwa Mgbanwe DNA Bisulfite

Enwere ike mezue ntụgharị na ịdị ọcha n'ime awa 2, yana ọnụego ntụgharị ruo 99%.

A na -emepụta Kit Nchigharị nke Bisulfite DNA maka ntụgharị bisulfite DNA na nyocha methylation DNA. Usoro ọgwụgwọ nke nlele DNA na -eji ngwa a nwere ike mezue n'ime awa 2, ọnụego mgbanwe nke cytosine na -enweghị methylated gaa uracil karịrị 99%. Ngwa a na -anabata ihe pụrụ iche nke onye na -echebe DNA, nke na -ekwe nkwa ịdị mma na mgbake nke DNA mgbe a tọghatara ya. Na mgbakwunye, ngwa a na-ejikwa usoro mwepụ otu acid sulphurous acid, nke na-eme ka usoro ọrụ ya niile dị mfe.

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Nlereanya Nnwale

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■ Dị Mfe na ngwa ngwa: Enwere ike mezue usoro ntụgharị na ịdị ọcha n'ime awa 2, yana obere ihe achọrọ maka ngwa na akụrụngwa, nke dabara maka ụlọ ọrụ nyocha na ọkwa niile.
Rate Ọnụego mgbanwe dị elu: Ọnụego mgbanwe nke cytosine na -enweghị methylated ka uracil na nlele DNA karịrị 99%.
Mmetụta dị elu: Ngwa a nwere ike hazie ihe nlele DNA ruru 500 pg wee ruo 2.5 μg.

Nkọwapụta

Ụdị: Mgbanwe Bisulfite
Ihe nlele DNA: 500 pg-2.5 μg
Ọnụego ntụgharị: > 99%
Oge ọrụ: ~ 2 awa
Ngwa: Ihe nlele DNA nke ngwa a haziri dabara maka methylation PCR/qPCR akọwapụtara, usoro na microarray.

Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)

Nke gara aga: Ngwa mwepụta TIANSeq mRNA

Osote: Ngwa Methylation-specipc PCR (MSP)

Ntọala 1: ọnụego ntụgharị nke "C" na -enweghị methy ka ọ bụrụ "U"
N'iji mkpụrụ ndụ ihe nketa mmadụ dị ka ihe nnwale, ọnụego mgbake nke nlele nwere ike iru 65%, yana oke nke "C" na "U" na -enweghị atụ nwere ike iru karịa 99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.