■ Kasị ọsọ ọsọ: Antibody gbanwetụrụ Anti Taq DNA polymerase, na -arụkọ ọrụ na sistemụ Buffer ngwa ngwa pụrụ iche, na -echekwa ihe ruru 60% nke oge mmeghachi omume wee bụrụ ngwa ngwa SYBR Green ngwa ngwa n'ahịa ugbu a.
■ Ike nkwalite siri ike: mgbaàmà mgbapụta ọkụ siri ike na -abawanye, nsonaazụ ziri ezi na ntụkwasị obi.
Stability Ezi nkwụsi ike: A na -agbakwunye ihe nkwụnye PCR pụrụ iche na nkwalite na nchekwa, na -eme ka nsonaazụ ya kwụsie ike, na -ekwughachi, ziri ezi na ntụkwasị obi.
Applicable A na -ahụkarị ya: Ọ bụghị naanị na ọ dabara maka ngwa PCR ngwa ngwa, kamakwa ọ dabara maka ngwa PCR nkịtị.
Ction Ndozi ROX: A na -ekekọta ihe mkpuchi ROX iche iche, nke na -eme mgbanwe karịa iji wee nwee ike hụ na nsonaazụ ziri ezi.
Pịnye: Polymerase DNA na-ebido ebido ebibi, SYBR Green I
Oge ọrụ: ~ 30 min
Ngwa: qPCR nke nwere agba maka nchọpụta mkpụrụ ndụ na DNA ma ọ bụ nyocha ngosipụta ikwu na ihe atụ cDNA sitere na ụdị dị iche iche.
Enwere ike ịhazi ngwaahịa niile maka ODM/OEM. Maka nkọwa,biko pịa Ọrụ ahaziri (ODM/OEM)
Ike nkwalite nkwalite siri ike- mgbaama fluorescence siri ike Ngwa ngwa-Meziwanye nnwale nnwale, gbatịkwuo ndụ ọrụ nke akụrụngwa wee chekwaa ume Taq Enwere ike ịgbalite DNA polymerase ngwa ngwa, na -eme ka ngwaahịa FastFire bụrụ ngwa ngwa SYBR Green n'ahịa ugbu a nwere sistemụ nchekwa ngwa ngwa pụrụ iche iji meziwanye nnwale nnwale ahụ. |
|
Ike nkwalite nkwalite siri ike- mgbaama fluorescence siri ike Ihe mgbaàmà fluorescence gbasawanyere siri ike (ikike ịgbasa ozi siri ike), yana usoro ịgbasawanye ọkọlọtọ, oke mmetụta, yana nwee ike ịchọpụta n'ụzọ ziri ezi na ọnụ ọgụgụ nke mkpụrụedemede ebumnuche nke ndebiri dị ala. Mgbaàmà nchọpụta ngwaahịa dị mkpa sitere na ndị na -ebunye T adịghị ike, uru CT na -ala azụ, nke nwere ike ibute nsonaazụ na -ezighi ezi. |
|
Nnwale ugboro ugboro nke ọma 96 na-egosi na reagent nwere nkwụsi ike siri ike yana ikwughachi ka mma. |
Kemgbe e guzobere ya, ụlọ ọrụ mmepụta ihe anyị na -emepụta ngwaahịa klaasị mbụ ụwa na ịgbaso ụkpụrụ
nke mma mbụ. Ngwaahịa anyị enwetala ezigbo aha na ụlọ ọrụ yana ntụkwasị obi bara uru n'etiti ndị ahịa ọhụrụ na ndị agadi ..